| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Landry, Zavin L (11)
|
NTNTN |
36.98 |
35.83 |
-1.15 |
| 2 |
Azarov, Alex G (12)
|
WTCOM |
38.10 |
36.79 |
-1.31 |
| 3 |
Erastov, Alexander (12)
|
NTNTN |
37.71 |
37.76 |
0.05 |
| 4 |
Zhang, William T (12)
|
NTNTN |
37.98 |
38.05 |
0.07 |
| 5 |
Isaacs-Sodeye, Judah I (11)
|
AZUN |
40.14 |
38.45 |
-1.69 |
| 6 |
Phillips, Ryan (12)
|
WTCOM |
42.07 |
40.88 |
-1.19 |
| 7 |
Wang, Richard C (11)
|
NTNTN |
43.95 |
41.21 |
-2.74 |
| 8 |
Heldenbrand, Dylan F (11)
|
NTNTN |
41.41 |
41.54 |
0.13 |
| 9 |
Sun, Alexander J (11)
|
NTNTN |
43.61 |
42.51 |
-1.10 |
| 10 |
Cai, Matthew (12)
|
NTNTN |
42.27 |
42.79 |
0.52 |
| 11 |
Hough, Logan H (12)
|
NTNTN |
46.05 |
44.19 |
-1.86 |
| 12 |
Janning, Joshua W (11)
|
NTNTN |
46.53 |
45.35 |
-1.18 |
| 13 |
Dollins, Preston C (11)
|
NTFNW |
48.21 |
46.63 |
-1.58 |
| 14 |
Farrera, Oscar J (11)
|
WTCOM |
48.37 |
47.82 |
-0.55 |
| 15 |
Andrews, Richard O (11)
|
NTBOSS |
54.28 |
47.96 |
-6.32 |
| 16 |
Stratton, Isaac R (11)
|
NTNTN |
48.95 |
48.93 |
-0.02 |
| 17 |
Kipp, Colt M (11)
|
NTNTN |
51.00 |
49.19 |
-1.81 |
| 18 |
Chupin, Toyo (12)
|
NTNTN |
48.95 |
50.03 |
1.08 |
| 19 |
Thota, Pranav (11)
|
NTO2 |
59.07 |
54.92 |
-4.15 |
|
Lee, Kevin T (11)
|
NTNTN |
|
NS |
- |