| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Klopfenstein, Sydney K (12)
|
NTNTN |
31.94 |
31.21 |
-0.73 |
| 2 |
Khalaf, Jenna A (11)
|
NTNTN |
33.56 |
31.33 |
-2.23 |
| 3 |
Aegerter, Gabrielle A (11)
|
NTNTN |
33.10 |
32.59 |
-0.51 |
| 4 |
Hays, Kenzi J (11)
|
NTFNW |
36.85 |
35.84 |
-1.01 |
| 5 |
Schultz, Riley E (11)
|
NTNTN |
37.70 |
36.79 |
-0.91 |
| 6 |
Reynolds, Brenna L (11)
|
NTUN |
37.78 |
37.52 |
-0.26 |
| 7 |
Woods, Rachel N (12)
|
NTNTN |
41.14 |
38.53 |
-2.61 |
| 8 |
Stubbs, Olivia F (12)
|
NTNTN |
45.84 |
42.04 |
-3.80 |
| 9 |
Dobbins, Laura C (11)
|
NTFNW |
42.12 |
44.01 |
1.89 |
| 10 |
DeFranco, Francesca M (11)
|
NTNTN |
|
44.85 |
- |
| 11 |
Duncan, Katelyn G (12)
|
NTFNW |
48.09 |
44.95 |
-3.14 |
| 12 |
Bedgood, Caylee R (12)
|
NTNTN |
43.97 |
45.34 |
1.37 |
| 13 |
Okafo, Nonyem J (11)
|
NTNTN |
48.43 |
47.06 |
-1.37 |
| 14 |
Castellino, Anisha (11)
|
NTNTN |
|
49.02 |
- |
| 15 |
Robins, Erin M (12)
|
NTO2 |
|
49.79 |
- |
| 16 |
Erasani, Riya P (11)
|
NTNTN |
|
51.53 |
- |
| 17 |
Brock, Madeleine R (12)
|
NTNTN |
49.51 |
52.00 |
2.49 |
| 18 |
Wolfmueller, Charlotte R (11)
|
WTCOM |
57.19 |
55.46 |
-1.73 |
| 19 |
Prabhu, Aditi N (12)
|
NTNTN |
|
1:00.41 |
- |
|
Plows, Taryn S (11)
|
NTNTN |
31.63 |
NS |
- |
|
Shanker, Riddhima (12)
|
NTNTN |
37.29 |
NS |
- |