| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Peters, Evan M (12)
|
NIGCOM |
43.37 |
40.64 |
-2.73 |
| 2 |
Kilchevskyy, Michael (11)
|
NIWEBS |
41.38 |
41.03 |
-0.35 |
| 3 |
Brett, Liam J (11)
|
NIPACK |
43.60 |
41.94 |
-1.66 |
| 4 |
Fan, Timothy (11)
|
NIPACK |
45.84 |
43.60 |
-2.24 |
| 5 |
Chasman, Aidan P (12)
|
NIVICT |
44.71 |
43.93 |
-0.78 |
| 6 |
Palmesano, Samuel P (12)
|
NIHAWK |
54.93 |
46.62 |
-8.31 |
| 7 |
Cho, Seojun (11)
|
NIHAWK |
48.64 |
47.02 |
-1.62 |
| 8 |
Law, Linus C (11)
|
NIHAWK |
|
47.10 |
- |
| 9 |
Werth, Nathan B (12)
|
NIGRSC |
|
47.89 |
- |
| 10 |
Fox, Johnathan M (11)
|
NISDSC |
56.59 |
53.78 |
-2.81 |
| 11 |
Gargano, Adam L (12)
|
NIHAST |
50.32 |
54.52 |
4.20 |
| 12 |
Passinault, Easton J (11)
|
NIGRSC |
57.96 |
56.33 |
-1.63 |
| 13 |
Quigley, Kian C (11)
|
NIGRSC |
1:02.41 |
1:00.56 |
-1.85 |
| 14 |
Voytynskyy, Petro (12)
|
NIGRSC |
|
1:20.33 |
- |
|
Geoca, Andrew K (11)
|
NISDSC |
1:05.27 |
DQ |
- |
|
Ballard, Mason D (11)
|
NIHAWK |
1:16.05 |
NS |
- |