| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carlton, Caleb D (10)
|
NIWEBS |
46.23 |
45.14 |
-1.09 |
| 2 |
Ray, Landon M (9)
|
NIVICT |
48.14 |
48.76 |
0.62 |
| 3 |
Ernst, Emmett J (10)
|
NIWEBS |
46.14 |
49.71 |
3.57 |
| 4 |
Frenzel, Jack W (10)
|
NIWEBS |
52.48 |
54.34 |
1.86 |
| 5 |
Parker, William A (9)
|
NIHAWK |
53.79 |
54.85 |
1.06 |
| 6 |
Maracle, Liam E (9)
|
NIGRSC |
|
56.20 |
- |
| 7 |
Refici, Zane S (10)
|
NISDSC |
59.36 |
57.92 |
-1.44 |
| 8 |
Ramsperger, Jacob T (10)
|
NISDSC |
1:03.32 |
59.05 |
-4.27 |
| 9 |
Valentino, Aiden M (9)
|
NIGRSC |
1:09.12 |
1:12.09 |
2.97 |
|
Tang, Zixian R (10)
|
NIPACK |
|
DQ |
- |
|
Thompson, Kendrick F (10)
|
NIRRST |
|
DQ |
- |
|
De Pedro, Shawn Z (9)
|
NIHAWK |
|
NS |
- |
|
Law, Cyrus C (9)
|
NIHAWK |
|
NS |
- |
|
Wu, Winston X (10)
|
NIHAWK |
58.17 |
NS |
- |
|
Orynych, Dennis P (9)
|
NIGRSC |
1:03.73 |
NS |
- |
|
Voytynskyy, Taras (9)
|
NIGRSC |
|
NS |
- |