| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wagner, Morgan E (11)
|
NIVICT |
36.43 |
33.03 |
-3.40 |
| 2 |
Prezyna, Zoey (11)
|
NIVICT |
35.03 |
33.93 |
-1.10 |
| 3 |
Skizim, Elena K (12)
|
NIWEBS |
34.61 |
34.40 |
-0.21 |
| 4 |
Huerta-Andrade, Isabella (12)
|
NIPACK |
36.19 |
36.60 |
0.41 |
| 5 |
Wethey, Morgane A (11)
|
NIVICT |
37.64 |
39.11 |
1.47 |
| 6 |
Kapral, Chloe H (12)
|
NIHAWK |
39.55 |
39.39 |
-0.16 |
| 7 |
Schwandt, Natalie K (11)
|
NIGRAT |
41.18 |
40.37 |
-0.81 |
| 8 |
Magnera, Sofia A (12)
|
NIWEBS |
39.93 |
40.45 |
0.52 |
| 9 |
Wagner, Keaghan J (11)
|
NISDSC |
42.54 |
42.84 |
0.30 |
| 10 |
Romano, Rita M (11)
|
NINEWW |
42.05 |
43.07 |
1.02 |
| 11 |
Kenny, Elisabeth A (12)
|
NIVICT |
43.92 |
43.19 |
-0.73 |
| 12 |
Crisco, Jacqueline E (11)
|
NIHAWK |
46.25 |
45.17 |
-1.08 |
| 13 |
Huerta-Andrade, Amanda (11)
|
NIPACK |
51.78 |
45.31 |
-6.47 |
| 14 |
Man, Surya T (11)
|
NIPACK |
49.65 |
45.48 |
-4.17 |
| 15 |
Bullock, Melanie M (11)
|
NINEWW |
53.69 |
49.71 |
-3.98 |
| 16 |
Provost, Makenzie L (11)
|
NIGRSC |
|
56.18 |
- |
| 17 |
Reinhard, Maria C (12)
|
NIGRSC |
1:05.53 |
1:03.79 |
-1.74 |
|
Levi, Noa (11)
|
NIHAWK |
43.54 |
40.58 |
-2.96 |
|
Hayes, Kaitlyn E (11)
|
NIGRSC |
|
DQ |
- |
|
Falbo, Gianna B (11)
|
NIGRSC |
|
DQ |
- |
|
McCarthy, Kelsey S (12)
|
NIHAWK |
43.77 |
NS |
- |
|
Nitchman, Melina E (11)
|
NIWEBS |
45.93 |
NS |
- |