| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Flake, Rilee D (8)
|
GUNEHA |
47.95 |
44.85 |
-3.10 |
| 2 |
Cavalcanti, Juliana D (8)
|
GUCFSC |
46.44 |
46.08 |
-0.36 |
| 3 |
Olson, Amelia E (8)
|
GUCFSC |
43.26 |
46.59 |
3.33 |
| 4 |
Armada, Isabella S (8)
|
GUPACK |
49.15 |
47.67 |
-1.48 |
| 5 |
Bell, Zyenna D (8)
|
GUESA |
53.10 |
49.40 |
-3.70 |
| 6 |
Wright, Mia M (8)
|
GUPACK |
|
53.68 |
- |
| 7 |
Nguyen, Tran (8)
|
GUCFSC |
|
56.12 |
- |
| 8 |
Davis, Mila B (8)
|
GUPACK |
|
1:01.36 |
- |
| 9 |
Munoz, Isabella M (8)
|
GUESA |
|
1:05.24 |
- |
| 10 |
Cannon, Kierra G (8)
|
GUESA |
1:10.18 |
1:06.16 |
-4.02 |
| 11 |
Shen, Arianna L (8)
|
GUCFSC |
|
1:08.53 |
- |
|
Brunkhorst, Addison G (8)
|
GUCFSC |
55.95 |
DQ |
- |
|
Weems, Brianna I (8)
|
GULIFE |
|
NS |
- |