| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McKnight, Henry E (9)
|
GUVSST |
41.02 |
35.98 |
-5.04 |
| 2 |
Yang, Ren (10)
|
GUVSST |
37.82 |
36.78 |
-1.04 |
| 3 |
Curran, Max F (10)
|
GUVSST |
37.14 |
38.32 |
1.18 |
| 4 |
White, William E (9)
|
GUPACK |
39.77 |
39.11 |
-0.66 |
| 5 |
Reyes-Coronel, Matthew H (9)
|
GUPACK |
43.24 |
40.31 |
-2.93 |
| 6 |
Biddlecome, Tyler J (9)
|
GUMARC |
44.74 |
40.79 |
-3.95 |
| 7 |
Goss, Benjamin Y (10)
|
GUPACK |
45.53 |
42.78 |
-2.75 |
| 8 |
Decena, Joshua Y (10)
|
GUPACK |
41.56 |
43.94 |
2.38 |
| 9 |
Lyons, Taylor J (10)
|
GUHUNT |
47.08 |
45.37 |
-1.71 |
| 10 |
Favela, David E (9)
|
GUHUNT |
49.29 |
46.26 |
-3.03 |
| 11 |
Nguyen, Clayton T (10)
|
GUPACK |
45.66 |
46.92 |
1.26 |
| 12 |
Singh, Victor H (9)
|
GUVSST |
49.43 |
48.83 |
-0.60 |
| 13 |
Dumas, Alex M (9)
|
GUMARC |
|
49.02 |
- |
| 14 |
Thomson, Charlie E (9)
|
GUVSST |
53.00 |
52.95 |
-0.05 |
| 15 |
Stephens, Cash W (10)
|
GUMARC |
|
54.13 |
- |
| 16 |
Piefer, Caleb A (9)
|
GUVSST |
|
54.29 |
- |
| 17 |
Duvall, Grayson A (9)
|
GUPACK |
|
54.88 |
- |
| 18 |
Morgan, Caelin B (10)
|
GUPACK |
58.30 |
55.05 |
-3.25 |
| 19 |
Hargrave, Benjamin M (10)
|
GUPACK |
|
1:08.33 |
- |
|
O'Donnell, Michael S (10)
|
GUHUNT |
|
DQ |
- |
|
Piefer, Cameron J (10)
|
GUVSST |
|
DQ |
- |
|
Villasenor, Jose L (9)
|
GUPACK |
52.08 |
NS |
- |