| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Suarez, Cruz L (10)
|
GUUN-V |
33.03 |
31.41 |
-1.62 |
| 2 |
Armada, Adrian S (10)
|
GUPACK |
37.80 |
37.67 |
-0.13 |
| 3 |
Kumar, Raoul G (9)
|
GU300 |
40.40 |
39.73 |
-0.67 |
| 4 |
Ellison, Hunter S (10)
|
GUPACK |
41.68 |
40.52 |
-1.16 |
| 5 |
Davila, Santiago (10)
|
GUPACK |
|
41.70 |
- |
| 6 |
LaPell, Ethan G (10)
|
GUPACK |
42.20 |
43.25 |
1.05 |
| 7 |
Goss, Benjamin Y (9)
|
GUPACK |
51.94 |
48.08 |
-3.86 |
| 8 |
Zhang, Haoyang A (9)
|
GUPACK |
|
48.79 |
- |
| 9 |
Hamm, Preston R (9)
|
GUPACK |
|
49.11 |
- |
| 10 |
Fish, Aiden J (10)
|
GUPACK |
52.01 |
50.08 |
-1.93 |
| 11 |
Alade, Joshua O (9)
|
GU300 |
|
50.67 |
- |
| 12 |
Davis, Owen L (10)
|
GUPACK |
47.52 |
50.91 |
3.39 |
| 13 |
Wildermuth, Benjamin R (10)
|
GUPACK |
57.51 |
52.53 |
-4.98 |
| 14 |
To, Noah C (10)
|
GUPACK |
|
54.95 |
- |
| 15 |
Eveland, Kyan T (9)
|
GUVSST |
|
57.56 |
- |
| 16 |
Colligan, Gage (9)
|
GUUNAT |
|
59.16 |
- |
| 17 |
Bell, Joseph C (9)
|
GUPACK |
1:05.62 |
59.61 |
-6.01 |
|
Larson-Hendricks, Anders R (10)
|
GUPACK |
|
DQ |
- |
|
Zhu, Harrison L (10)
|
GUPACK |
1:02.20 |
NS |
- |
|
Ferrin Agra, Tome' A (9)
|
GUPACK |
|
NS |
- |