| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Holder, Aislinn G (15)
|
GUUN-S |
57.24 |
59.97 |
2.73 |
| 2 |
Jaco, Reagan L (15)
|
GUSSAN |
56.35 |
1:00.77 |
4.42 |
| 3 |
Byrd, Audrey A (16)
|
GUSST |
1:01.43 |
1:03.21 |
1.78 |
| 4 |
Moriarty, Kiley M (17)
|
GUSSAN |
1:02.91 |
1:04.75 |
1.84 |
| 5 |
Riederer, Aisling S (17)
|
GUSSAN |
1:01.68 |
1:04.79 |
3.11 |
| 6 |
Pfeifer, Abigail R (16)
|
GUSSAN |
1:01.41 |
1:05.06 |
3.65 |
| 7 |
Springett, Adrianna M (16)
|
GUSSAN |
1:08.77 |
1:06.66 |
-2.11 |
| 8 |
Beardmore, Ashley K (15)
|
GUUN-S |
1:07.58 |
1:08.28 |
0.70 |
| 9 |
Saria, Noelle D (18)
|
GUTYES |
|
1:10.30 |
- |
| 10 |
Kollatschny, Hannah E (15)
|
GUSSAN |
1:07.04 |
1:10.60 |
3.56 |
|
Caldwell, Isabelle N (15)
|
GUSSAN |
1:02.14 |
NS |
- |
|
Longbottom, Charlotte V (16)
|
GUUN-S |
|
NS |
- |