| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stanford, Sydney R (17)
|
GUUN-S |
23.50 |
24.74 |
1.24 |
| 2 |
Jaco, Reagan L (15)
|
GUSSAN |
24.49 |
25.62 |
1.13 |
| 3 |
Holder, Aislinn G (15)
|
GUUN-S |
24.32 |
25.66 |
1.34 |
| 4 |
Riederer, Muirenn F (15)
|
GUSSAN |
26.15 |
26.25 |
0.10 |
| 5 |
Moriarty, Kiley M (17)
|
GUSSAN |
25.24 |
26.49 |
1.25 |
| 6 |
Byrd, Audrey A (16)
|
GUSST |
24.87 |
26.53 |
1.66 |
| 7 |
Klawiter, Riley C (17)
|
GUSSAN |
25.44 |
26.90 |
1.46 |
| 8 |
LeBlanc, Olivia M (16)
|
GUSSAN |
26.66 |
27.61 |
0.95 |
| 9 |
Roth, Kayla M (15)
|
GUTYES |
27.43 |
27.66 |
0.23 |
| 10 |
Riederer, Aisling S (17)
|
GUSSAN |
26.95 |
27.72 |
0.77 |
| 11 |
Kohl, Abby L (16)
|
GUUN-S |
29.29 |
27.81 |
-1.48 |
| 12 |
Saria, Noelle D (18)
|
GUTYES |
|
28.31 |
- |
| 13 |
Ford, Dawn C (15)
|
GUSST |
28.48 |
28.66 |
0.18 |
| 14 |
Springett, Adrianna M (16)
|
GUSSAN |
28.15 |
30.34 |
2.19 |
|
Doyle, Jenna L (17)
|
GUTYES |
27.43 |
NS |
- |
|
Longbottom, Charlotte V (16)
|
GUUN-S |
24.28 |
NS |
- |