| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kahl, Cullen S (9)
|
GUVSST |
47.19 |
41.58 |
-5.61 |
| 2 |
Zhang, Haoyang A (10)
|
GUPACK |
48.69 |
45.84 |
-2.85 |
| 3 |
White, William E (9)
|
GUPACK |
47.80 |
47.63 |
-0.17 |
| 4 |
Reyes-Coronel, Matthew H (9)
|
GUPACK |
54.67 |
48.29 |
-6.38 |
| 5 |
Bell, Joseph C (10)
|
GUPACK |
47.16 |
49.16 |
2.00 |
| 6 |
Decena, Joshua Y (10)
|
GUPACK |
1:01.83 |
51.77 |
-10.06 |
| 7 |
Piefer, Cameron J (10)
|
GUVSST |
|
52.18 |
- |
| 8 |
Biddlecome, Tyler J (9)
|
GUMARC |
54.42 |
52.76 |
-1.66 |
| 9 |
Lin, Alexander (10)
|
GUPACK |
|
53.37 |
- |
| 10 |
Goss, Benjamin Y (10)
|
GUPACK |
58.95 |
53.61 |
-5.34 |
| 11 |
Johnson, Noah A (9)
|
GUVSST |
|
53.76 |
- |
| 12 |
Singh, Victor H (9)
|
GUVSST |
56.87 |
54.02 |
-2.85 |
| 13 |
Villasenor, Jose L (9)
|
GUPACK |
57.40 |
54.04 |
-3.36 |
| 14 |
Mayes, Martin R (9)
|
GUHUNT |
|
54.61 |
- |
| 15 |
Lomax, Colt J (9)
|
GUSTA |
1:07.95 |
55.18 |
-12.77 |
| 16 |
Lyons, Taylor J (10)
|
GUHUNT |
1:01.06 |
55.96 |
-5.10 |
| 17 |
Duvall, Grayson A (9)
|
GUPACK |
|
57.22 |
- |
| 18 |
Hargrave, Benjamin M (10)
|
GUPACK |
|
58.78 |
- |
| 19 |
Morgan, Caelin B (10)
|
GUPACK |
|
1:04.22 |
- |
| 20 |
Chandler, William S (9)
|
GUPACK |
|
1:16.37 |
- |
|
Thomson, Charlie E (9)
|
GUVSST |
|
NS |
- |