| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Edwards, Sophia C (12)
|
PVMACH |
33.79 |
33.59 |
-0.20 |
| 2 |
Evanko, Lila E (12)
|
PVFXFX |
35.61 |
35.22 |
-0.39 |
| 3 |
Gradich, Allison R (11)
|
PVNCAP |
36.75 |
35.68 |
-1.07 |
| 4 |
Hiller, Maika E (12)
|
PVMACH |
38.62 |
37.07 |
-1.55 |
| 5 |
Yasrebi, Ava L (11)
|
PVMACH |
37.63 |
37.43 |
-0.20 |
| 6 |
Kay, Samantha R (12)
|
PVNCAP |
40.54 |
38.40 |
-2.14 |
| 7 |
Pangera, Lakshmi Samanvi (11)
|
PVNCAP |
41.07 |
39.64 |
-1.43 |
| 8 |
Pape, Kathryn R (11)
|
PVNCAP |
40.07 |
40.34 |
0.27 |
|
Monterrey, Daniella J (11)
|
PVFAST |
51.99 |
NS |
- |
|
Lay, Kamryn J (11)
|
PVMACH |
35.58 |
NS |
- |
|
Marupaka, Shivani (11)
|
PVNCAP |
51.05 |
NS |
- |
|
Roy, Shifanjali G (11)
|
PVNCAP |
36.47 |
NS |
- |