| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lankford, Theresa M (13)
|
MDLBA |
2:28.87 |
2:30.74 |
1.87 |
| 2 |
Thornton, Kira N (13)
|
PVMACH |
2:37.85 |
2:39.41 |
1.56 |
| 3 |
Huffstutler, Anabel H (14)
|
PVMACH |
2:33.45 |
2:41.53 |
8.08 |
| 4 |
Feracci, Madison E (14)
|
MDLBA |
2:38.26 |
2:44.14 |
5.88 |
| 5 |
Janczyk, Megan D (13)
|
PVMACH |
2:45.60 |
2:45.32 |
-0.28 |
| 6 |
Callahan, Grace H (13)
|
PVMACH |
|
2:47.25 |
- |
| 7 |
Fish, Lillianne E (14)
|
PVPM |
2:45.63 |
2:48.23 |
2.60 |
| 8 |
Gilbert, Samantha R (13)
|
PVMACH |
2:43.78 |
2:53.90 |
10.12 |
| 9 |
Jones, Ellie G (13)
|
PVMACH |
2:53.31 |
2:56.65 |
3.34 |
| 10 |
Knaggs, Claire E (14)
|
PVMACH |
2:46.61 |
2:57.28 |
10.67 |
| 11 |
O'Sullivan, Anna F (14)
|
PVMACH |
2:54.52 |
2:57.34 |
2.82 |
| 12 |
Bodner, Leila G (13)
|
PVMACH |
2:55.21 |
2:57.48 |
2.27 |
| 13 |
Caicedo, Haley A (13)
|
PVMACH |
2:56.03 |
2:58.19 |
2.16 |
| 14 |
Norris, Hayley C (14)
|
PVPM |
3:03.14 |
3:01.38 |
-1.76 |
| 15 |
Gonyea, Lucia M (14)
|
PVMACH |
3:03.44 |
3:05.92 |
2.48 |
| 16 |
Ferat, Katherine A (13)
|
PVMACH |
3:10.50 |
3:09.66 |
-0.84 |
| 17 |
Lombardi, Alexandra S (13)
|
PVMACH |
|
3:13.35 |
- |
| 18 |
Williams, Alexis E (13)
|
PVMACH |
3:13.90 |
3:15.94 |
2.04 |
| 19 |
Wevley, Grace C (14)
|
PVMACH |
3:19.68 |
3:19.19 |
-0.49 |
|
Annis, Kelsey L (14)
|
MDLBA |
3:18.48 |
DQ |
- |