| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lam, Samuel J (10)
|
PVMACH |
39.71 |
38.72 |
-0.99 |
| 2 |
Phillips, Benjamin M (10)
|
PVMACH |
42.88 |
39.38 |
-3.50 |
| 3 |
Schrock, Eamon C (10)
|
PVSTJS |
42.10 |
40.14 |
-1.96 |
| 4 |
Warren, Charles T (10)
|
PVMACH |
41.21 |
40.61 |
-0.60 |
| 5 |
Braccia, Samuel K (10)
|
PVMACH |
41.07 |
42.12 |
1.05 |
| 6 |
Abney, Luke G (10)
|
PVMACH |
41.49 |
43.35 |
1.86 |
| 7 |
Montgomery, Nicholas S (10)
|
PVSTJS |
43.32 |
43.57 |
0.25 |
| 8 |
Franklin, Marius M (10)
|
PVMACH |
45.26 |
43.58 |
-1.68 |
| 9 |
Alami, Zakaria O (10)
|
PVMACH |
46.78 |
46.01 |
-0.77 |
| 10 |
Dalton, Caiden W (10)
|
PVMACH |
49.57 |
48.27 |
-1.30 |
| 11 |
Abney, Jake G (10)
|
PVMACH |
49.15 |
49.62 |
0.47 |
| 12 |
Christman, Tomas J (10)
|
PVSTJS |
47.38 |
50.65 |
3.27 |
| 13 |
Chase, Henry J (10)
|
PVSTJS |
|
51.84 |
- |
| 14 |
Taylor, Matthew T (10)
|
PVMACH |
56.60 |
53.64 |
-2.96 |
| 15 |
Meyers, John A (10)
|
PVMACH |
58.98 |
1:01.81 |
2.83 |
|
Lee, Benjamin I (10)
|
PVMACH |
53.11 |
NS |
- |
|
Black, Jaylen R (10)
|
PVMACH |
1:02.46 |
NS |
- |