| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Roose, Ralf (19)
|
PVGW |
2:20.09 |
2:18.55 |
-1.54 |
| 2 |
Sosnowski, Benjamin T (18)
|
UW-G |
2:30.76 |
2:25.69 |
-5.07 |
| 3 |
Rodgers, Connor C (22)
|
PVGW |
2:31.18 |
2:27.64 |
-3.54 |
| 4 |
Groves, Cameron C (16)
|
PVASA |
2:41.94 |
2:39.41 |
-2.53 |
| 5 |
Murphy, Taiyo E (16)
|
PVASA |
2:50.83 |
2:43.19 |
-7.64 |
| 6 |
Chen, Kyle T (15)
|
PVASA |
2:48.12 |
2:44.47 |
-3.65 |
| 7 |
McGregor, Austyn J (18)
|
PVMACH |
2:32.06 |
2:44.95 |
12.89 |
| 8 |
Ramsdell, Jonathan C (16)
|
PVNCAP |
2:49.01 |
2:45.47 |
-3.54 |
| 9 |
Jones, Olaoluwadeji E (17)
|
PVMACH |
2:40.70 |
2:48.64 |
7.94 |
| 10 |
Cobb, Owen C (16)
|
PVMACH |
2:53.09 |
2:52.72 |
-0.37 |
| 11 |
Pahlevanpour, Alex N (16)
|
PVMACH |
3:04.00 |
2:53.76 |
-10.24 |
| 12 |
Lukin, Myroslav (16)
|
PVMACH |
2:56.25 |
2:55.02 |
-1.23 |
| 13 |
Finnegan, Collin A (16)
|
PVNCAP |
|
2:56.03 |
- |
| 14 |
Ledyashov, Sergey D (16)
|
PVMACH |
2:21.13 |
2:56.23 |
35.10 |
| 15 |
Koester, Griffin H (18)
|
PVMACH |
|
2:57.50 |
- |
| 16 |
Nam, Jayden K (15)
|
PVMACH |
2:54.21 |
2:57.51 |
3.30 |
| 17 |
Vaughn, Jordan Q (16)
|
PVMACH |
3:09.58 |
2:59.56 |
-10.02 |
| 18 |
Center, William H (16)
|
PVMACH |
2:57.10 |
3:00.87 |
3.77 |
| 19 |
Cunningham-Bennett, Aidan T (17)
|
PVMACH |
3:08.97 |
3:03.29 |
-5.68 |
| 20 |
Zhang, Bryan Y (15)
|
PVMACH |
3:26.61 |
3:03.59 |
-23.02 |
| 21 |
Nigri, Noah D (15)
|
PVNCAP |
3:05.56 |
3:06.15 |
0.59 |
| 22 |
Nguyen, Blake R (15)
|
PVMACH |
3:13.60 |
3:06.53 |
-7.07 |
| 23 |
Chavez, Marco A (15)
|
PVMACH |
3:11.89 |
3:13.01 |
1.12 |
| 25 |
Eschmann, John F (16)
|
PVRY |
|
3:25.50 |
- |
| 26 |
Childs, Michener E (15)
|
PVMACH |
3:53.55 |
3:34.89 |
-18.66 |
|
Kaplan, Ari J (17)
|
PVMACH |
2:40.56 |
NS |
- |