| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Li, Chelsea T (8)
|
VAECAT |
1:00.13 |
41.18 |
-18.95 |
| 2 |
Kasa, Keira L (8)
|
NCYBAC |
44.55 |
42.52 |
-2.03 |
| 3 |
Upton, Camryn M (8)
|
NCYBAC |
41.97 |
43.49 |
1.52 |
| 4 |
Karasiewicz, Hayden A (7)
|
MDBAY |
38.62 |
43.84 |
5.22 |
| 5 |
Phommachanh, Macy T (8)
|
VAPWSC |
52.71 |
44.61 |
-8.10 |
| 6 |
Eddy, Piper R (7)
|
VARAYS |
44.28 |
46.89 |
2.61 |
| 7 |
Knerr, Lillianne G (7)
|
VARAYS |
1:13.55 |
53.90 |
-19.65 |
| 8 |
Howell, Kira B (8)
|
VARAYS |
54.62 |
54.05 |
-0.57 |
| 9 |
McMunn, Presley R (8)
|
VAPSDN |
58.52 |
54.10 |
-4.42 |
| 10 |
Connolly, Scarlett F (8)
|
VARAYS |
49.69 |
54.33 |
4.64 |
| 11 |
Bertoldi, Jillian M (7)
|
VARAYS |
54.04 |
56.20 |
2.16 |
| 12 |
Lewis, Reagan Q (8)
|
MDBAY |
52.97 |
56.56 |
3.59 |
| 13 |
Tricoli, Ava T (6)
|
VAHNVR |
48.59 |
59.02 |
10.43 |
| 14 |
Frederick, Samantha V (7)
|
VAPWSC |
1:03.27 |
1:05.99 |
2.72 |
| 15 |
Bernal-Garcia, Daniella (7)
|
VAPWSC |
1:02.99 |
1:13.19 |
10.20 |
|
Crone, Hayes T (8)
|
VANOVA |
36.99 |
NS |
- |
|
Snead, Aaliyah M (7)
|
VAPWSC |
1:00.59 |
NS |
- |