| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Webster, Olivia Y (15)
|
VAWST |
33.12 |
28.72 |
-4.40 |
| 2 |
Stevens, Emily G (14)
|
VAHNVR |
31.27 |
29.69 |
-1.58 |
| 3 |
Reed, Madison N (13)
|
VAVSTP |
29.98 |
30.04 |
0.06 |
| 4 |
Tanner, Kiersten N (18)
|
VAVSTP |
31.78 |
30.33 |
-1.45 |
| 5 |
Douglas, Gabriella M (14)
|
VANOVA |
31.12 |
30.38 |
-0.74 |
| 6 |
Rosario, Nicole (16)
|
PVYASD |
34.05 |
31.30 |
-2.75 |
| 7 |
Tanner, Kaley A (13)
|
VAVSTP |
35.08 |
31.33 |
-3.75 |
| 8 |
Lindsey, Isabelle K (13)
|
VAHNVR |
31.76 |
31.48 |
-0.28 |
| 9 |
Defibaugh, Gracie L (13)
|
VAVSTP |
33.66 |
32.93 |
-0.73 |
| 10 |
Curry, Kathleen (18)
|
PVYASD |
|
34.08 |
- |
| 11 |
Beckett, Audrey S (13)
|
VAHNVR |
|
34.57 |
- |
| 12 |
Davis, Elizabeth C (14)
|
VANOVA |
34.45 |
35.51 |
1.06 |
| 13 |
Coward, Elisabeth H (17)
|
VAHNVR |
35.91 |
38.71 |
2.80 |
| 14 |
Cerny, Maclay T (14)
|
VANOVA |
43.06 |
40.62 |
-2.44 |
|
Twigg, Madelynn A (14)
|
VAVSTP |
30.08 |
NS |
- |
|
Rollf, Shelbey P (14)
|
VANOVA |
42.27 |
NS |
- |
|
Jarrett-Peace, Kaydence A (13)
|
VANOVA |
33.47 |
NS |
- |