| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fulton, Alexa L (14)
|
MAUMLY |
24.10 |
23.97 |
-0.13 |
| 2 |
Trietley, Alexis C (14)
|
NIBRAD |
24.20 |
24.08 |
-0.12 |
| 3 |
Lowe, Madelyn G (13)
|
MAKAY |
24.29 |
24.31 |
0.02 |
| 4 |
Simpson, Katherine A (14)
|
MAWCAY |
24.37 |
24.32 |
-0.05 |
| 5 |
Gowetski, JJ (13)
|
MYST |
24.72 |
24.42 |
-0.30 |
| 6 |
Gring, Sydney (13)
|
MARY |
24.29 |
24.51 |
0.22 |
| 7 |
Schermerhorn, Maren M (14)
|
MABYNS |
24.81 |
24.77 |
-0.04 |
| 8 |
Cherrie, Emily (14)
|
MARY |
24.96 |
24.92 |
-0.04 |
| 9 |
Neiss, Anna R (14)
|
AMRESY |
24.87 |
24.93 |
0.06 |
| 10 |
Scott, Adeleigh (14)
|
MAJY |
25.50 |
24.96 |
-0.54 |
| 11 |
Hofmann, Lina A (14)
|
MACBFY |
25.67 |
25.01 |
-0.66 |
| 12 |
Ciez, Ella R (13)
|
AMUNTY |
25.16 |
25.30 |
0.14 |
| 13 |
Fenn, Victoria A (14)
|
MAPFY |
25.37 |
25.38 |
0.01 |
| 14 |
Carney, Kylie M (14)
|
MARAYS |
25.43 |
25.46 |
0.03 |
| 15 |
Kubiak, Mary J (13)
|
MARAYS |
25.65 |
25.67 |
0.02 |
| 16 |
Lippert, Catherine J (13)
|
ZZCYAA |
25.84 |
25.69 |
-0.15 |
| 17 |
Dagg, Kiersten A (14)
|
MAPAY |
25.87 |
25.73 |
-0.14 |
| 18 |
Philbrick, Olivia L (14)
|
MYST |
25.40 |
25.74 |
0.34 |
| 19 |
Strine, Jillian C (13)
|
MACC |
25.54 |
25.77 |
0.23 |
| 20 |
Freedman, Rebecca J (14)
|
MAWSY |
26.02 |
25.96 |
-0.06 |
| 21 |
Murphy, Jillian E (13)
|
MAKAY |
25.77 |
26.07 |
0.30 |
| 22 |
Chang, Lauren M (14)
|
MAWSY |
26.20 |
26.10 |
-0.10 |
| 23 |
Barton, Ava (13)
|
RGS |
25.96 |
26.11 |
0.15 |
| 24 |
Yuengling, Morgan L (14)
|
MASKY |
26.11 |
26.26 |
0.15 |