| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hetrick, Paige M (16)
|
NIBRAD |
23.04 |
23.25 |
0.21 |
| 2 |
Brown, Rebecca M (18)
|
MARY |
23.76 |
23.85 |
0.09 |
| 3 |
Mostek, Anya H (15)
|
MAPAY |
24.35 |
24.19 |
-0.16 |
| 4 |
Strunk, Skylar R (16)
|
MABYNS |
24.33 |
24.24 |
-0.09 |
| 5 |
Fresa, Noel J (16)
|
MAUPY |
24.44 |
24.40 |
-0.04 |
| 6 |
Walting, Margaret E (16)
|
MAWBY |
24.56 |
24.64 |
0.08 |
| 7 |
Matthews, Madison (18)
|
FLY |
24.48 |
24.69 |
0.21 |
| 8 |
Zelinske, Alexandra B (16)
|
MAPAY |
24.99 |
24.72 |
-0.27 |
| 8 |
Kinton, Madeline (15)
|
MAWAY |
24.84 |
24.72 |
-0.12 |
| 10 |
Cunningham, Rachel L (15)
|
MAHAY |
24.50 |
24.77 |
0.27 |
| 11 |
Campbell, Cailin E (18)
|
MAPFY |
24.60 |
24.78 |
0.18 |
| 12 |
Wasielevski, Caroline (17)
|
MARY |
24.95 |
24.88 |
-0.07 |
| 13 |
Roerig, Skylar (15)
|
MAWBY |
25.06 |
24.89 |
-0.17 |
| 14 |
Fenn, Lauren E (17)
|
MAPFY |
24.69 |
24.96 |
0.27 |
| 15 |
Leskovansky, Abigail M (15)
|
CLFD |
25.42 |
25.20 |
-0.22 |
| 16 |
Lahmann-Metcalfe, Helen M (16)
|
MARY |
25.21 |
25.21 |
0.00 |
| 17 |
Ritter, Sarah G (15)
|
MAPAY |
25.02 |
25.38 |
0.36 |
| 18 |
Brattley, Allison (16)
|
MATRVY |
25.30 |
25.39 |
0.09 |
| 19 |
Roscoe, Jenna M (16)
|
AMUNTY |
25.25 |
25.57 |
0.32 |
| 20 |
BecK, Zoey R (15)
|
AMGCY |
25.30 |
25.60 |
0.30 |
| 21 |
Mikesell, Raegan F (16)
|
CLFD |
25.75 |
25.66 |
-0.09 |
| 22 |
Feiertag, Greta A (16)
|
MABYNS |
25.37 |
25.74 |
0.37 |
| 23 |
McNulty, Peyton (18)
|
MAPFY |
25.41 |
25.77 |
0.36 |
| 24 |
Yuan, Allison M (16)
|
MAUMLY |
25.31 |
25.88 |
0.57 |