| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harms, Stella J (7)
|
PVNCAP |
25.43 |
24.61 |
-0.82 |
| 2 |
Matthews, Aubree L (7)
|
PVNCAP |
27.45 |
25.83 |
-1.62 |
| 3 |
Routman, Kelsey A (7)
|
PVBWST |
28.11 |
27.83 |
-0.28 |
| 4 |
Dufault, Audrey A (7)
|
PVNCAP |
33.58 |
27.86 |
-5.72 |
| 5 |
Lucchese, Alexandra G (7)
|
PVNCAP |
27.23 |
28.51 |
1.28 |
| 6 |
Weliwitigoda, Mila A (7)
|
PVNCAP |
28.45 |
28.67 |
0.22 |
| 7 |
Shoemaker, Madeline G (7)
|
PVNCAP |
30.93 |
29.74 |
-1.19 |
| 8 |
Charlton, Audrey L (7)
|
PVNCAP |
30.24 |
29.82 |
-0.42 |
| 9 |
Farmer, Morgan M (7)
|
PVSNOW |
33.20 |
29.98 |
-3.22 |
| 10 |
Roy, Shifanjali G (6)
|
PVNCAP |
28.20 |
30.36 |
2.16 |
| 11 |
Wong, Arianna K (6)
|
PVSNOW |
30.57 |
30.53 |
-0.04 |
| 12 |
Lynch, Rylie M (7)
|
PVSNOW |
29.88 |
30.76 |
0.88 |
| 13 |
Demissie, Hannah B (6)
|
PVNCAP |
32.64 |
31.14 |
-1.50 |
| 14 |
Kibret, Eldhana B (7)
|
PVNCAP |
31.00 |
31.75 |
0.75 |
| 15 |
Light, Willow R (6)
|
PVNCAP |
|
31.91 |
- |
| 16 |
Gager, Marleejane M (7)
|
PVNCAP |
35.74 |
32.59 |
-3.15 |
| 17 |
Richards, Ella M (7)
|
PVNCAP |
34.55 |
33.06 |
-1.49 |
| 18 |
Curran, Katherine A (7)
|
PVSNOW |
40.67 |
34.53 |
-6.14 |
| 19 |
Shive, Keira X (7)
|
PVNCAP |
38.15 |
34.89 |
-3.26 |
| 20 |
Detrick, Emma M (7)
|
PVNCAP |
34.57 |
35.36 |
0.79 |
| 21 |
Arekal, Adya N (7)
|
PVBWST |
|
37.22 |
- |
| 22 |
Nugent, Emily J (7)
|
PVNCAP |
|
37.56 |
- |
| 23 |
Dunn, Olive M (6)
|
PVNCAP |
40.58 |
37.83 |
-2.75 |
| 24 |
Jung, Alina Y (7)
|
PVNCAP |
35.91 |
38.11 |
2.20 |
| 25 |
Miceli, Charlotte J (7)
|
PVBWST |
|
39.01 |
- |
| 26 |
Ferner, Mia E (7)
|
PVBWST |
|
39.11 |
- |
| 27 |
Allen, Addison G (7)
|
PVNCAP |
41.76 |
41.11 |
-0.65 |
|
Skidan, Maria (6)
|
PVHACC |
|
DQ |
- |