| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Howard, Charles P (10)
|
FGJDST |
45.20 |
44.88 |
-0.32 |
| 2 |
Gonzalez, Manuel S (10)
|
FGMART |
47.13 |
45.44 |
-1.69 |
| 3 |
Lilienthal, Robert W (10)
|
FGECAC |
46.26 |
46.88 |
0.62 |
| 4 |
Lee, Steven (9)
|
FGWOW |
48.89 |
47.02 |
-1.87 |
| 5 |
Wasielewski, Nicolas M (9)
|
FGMART |
53.63 |
49.06 |
-4.57 |
| 6 |
Patel, Yogi (9)
|
FGWOW |
50.76 |
49.92 |
-0.84 |
| 7 |
Scholl, Hayden J (9)
|
FGJDST |
48.53 |
50.36 |
1.83 |
| 8 |
Shelton, Erick A (10)
|
FGMART |
59.64 |
50.73 |
-8.91 |
| 9 |
Landa, Alex (9)
|
FGWOW |
56.37 |
51.01 |
-5.36 |
| 10 |
Bollella, Nico D (10)
|
FGECAC |
57.31 |
53.68 |
-3.63 |
| 11 |
Kennedy, Kai M (9)
|
FGJDST |
|
57.01 |
- |
| 12 |
Rottcher, Benjamin (9)
|
FGWOW |
57.30 |
58.96 |
1.66 |
| 13 |
Howell, Bo H (10)
|
FGWOW |
57.81 |
1:04.26 |
6.45 |
| 14 |
Holland, Cole H (10)
|
FGMART |
|
1:05.40 |
- |
|
Martinez, Andric E (10)
|
FGWOW |
1:05.75 |
NS |
- |