| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tremaine, Malea T (12)
|
GUESCA |
39.93 |
39.66 |
-0.27 |
| 2 |
Childers, Madison N (11)
|
GUUN-B |
40.11 |
40.42 |
0.31 |
| 3 |
Masri, Violet G (11)
|
GUAGS |
41.72 |
41.88 |
0.16 |
| 4 |
Miksenaite, Rugile E (12)
|
GUKATY |
39.93 |
42.95 |
3.02 |
| 5 |
Baker, Brooke C (12)
|
GUESCA |
43.53 |
43.66 |
0.13 |
| 6 |
Pham, Elizabeth A (12)
|
GUESCA |
44.15 |
43.74 |
-0.41 |
| 7 |
Ly, Yvonne K (11)
|
GUESCA |
44.00 |
44.74 |
0.74 |
| 8 |
Madden, Jasmine J (11)
|
GURICE |
47.05 |
45.61 |
-1.44 |
| 9 |
Derby, Bella V (12)
|
GUPFL |
39.29 |
45.73 |
6.44 |
| 10 |
Makasheva, Kamila (11)
|
GURICE |
46.64 |
46.15 |
-0.49 |
| 11 |
Hwang, Abigail Y (12)
|
GUKATY |
46.68 |
46.27 |
-0.41 |
| 12 |
York, Emily A (12)
|
GURICE |
40.69 |
46.46 |
5.77 |
| 13 |
Wedlick, Keira L (12)
|
GURICE |
46.64 |
47.02 |
0.38 |
| 14 |
Solis, Gracie E (12)
|
GUKATY |
|
48.11 |
- |
| 15 |
Glascock, Sara E (12)
|
GUKATY |
46.15 |
48.41 |
2.26 |
| 16 |
Doumit Isaza, Andrea N (12)
|
GUTASC |
47.86 |
48.43 |
0.57 |
| 17 |
Kumar, Anita S (11)
|
GUKATY |
56.29 |
48.88 |
-7.41 |
| 18 |
Bergman, Taylor J (11)
|
GURICE |
44.32 |
50.02 |
5.70 |
| 19 |
Demlow, Miriam E (12)
|
GUAGS |
52.98 |
50.18 |
-2.80 |
| 20 |
Yang, Maggie J (11)
|
GUKATY |
56.21 |
50.53 |
-5.68 |
| 21 |
Peden, Ashlee C (12)
|
GUKATY |
47.69 |
51.59 |
3.90 |
| 22 |
Mendez, Ava N (12)
|
GUPACK |
48.29 |
51.69 |
3.40 |
| 23 |
Chau, Eliana H (11)
|
GUKATY |
54.31 |
51.83 |
-2.48 |
| 24 |
Friedman, Emma G (11)
|
GUSPA |
52.72 |
51.92 |
-0.80 |
| 25 |
Field, Claire M (12)
|
GURICE |
45.14 |
52.08 |
6.94 |
| 26 |
Beery, Daniela N (11)
|
GUPACK |
47.75 |
52.89 |
5.14 |
| 27 |
Rajanna, Krisha (11)
|
GUKATY |
1:02.15 |
54.81 |
-7.34 |
| 28 |
Payne, Addison R (11)
|
GUMARC |
1:07.74 |
56.63 |
-11.11 |