| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Siegler, Jamie R (20)
|
GUUN-B |
2:55.11 |
2:57.31 |
2.20 |
| 2 |
Gottfried, Charlotte A (16)
|
GUPACK |
3:03.34 |
3:04.47 |
1.13 |
| 3 |
Criscitiello, Sydney L (17)
|
GUAGS |
2:57.06 |
3:04.84 |
7.78 |
| 4 |
Lu, Sarah (15)
|
GUKATY |
2:55.06 |
3:06.04 |
10.98 |
| 5 |
Baron, Jordan E (15)
|
GUBATS |
3:09.52 |
3:06.24 |
-3.28 |
| 6 |
Goebel, Riley K (15)
|
GUBTA |
3:11.40 |
3:08.88 |
-2.52 |
| 7 |
Kohl, Abby L (17)
|
GUSSAN |
3:09.52 |
3:12.89 |
3.37 |
| 8 |
Diara, Tessa F (15)
|
GURICE |
3:07.44 |
3:14.22 |
6.78 |
| 9 |
Singh, Isabelle H (15)
|
GUPACK |
3:12.11 |
3:15.05 |
2.94 |
| 10 |
Bernal Gomez, Paula A (16)
|
GUAGS |
|
3:20.70 |
- |
| 11 |
Sansom, Caroline J (16)
|
GURICE |
3:36.44 |
3:21.34 |
-15.10 |
| 12 |
Abrego, Kaylen J (15)
|
GUPACK |
3:22.75 |
3:26.05 |
3.30 |
| 13 |
O'Sullivan, Mary K (16)
|
GUPACK |
3:32.44 |
3:28.33 |
-4.11 |
| 14 |
Woodhouse, Hannah (15)
|
GURICE |
3:33.70 |
3:36.37 |
2.67 |
| 15 |
Musselman, Madison P (15)
|
GUKATY |
3:33.59 |
3:39.69 |
6.10 |
| 16 |
Knoerzer, Lexi L (15)
|
GUBTA |
|
3:48.02 |
- |