| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Warfield, McKayla L (10)
|
GUHCAP |
42.85 |
40.20 |
-2.65 |
| 2 |
Anzaldua, Lily A (10)
|
GUSCAT |
48.07 |
40.89 |
-7.18 |
| 3 |
Warfield, Kaylee A (10)
|
GUPEAK |
55.71 |
50.48 |
-5.23 |
| 4 |
Dowell, Ada G (10)
|
GUAQUA |
|
51.28 |
- |
| 5 |
Hernandez, Allisson S (10)
|
GUNOCH |
56.52 |
54.82 |
-1.70 |
| 6 |
Needham, Isabella K (9)
|
GUPEAK |
|
1:08.05 |
- |
|
Bhatt, Shraya S (10)
|
GUAQUA |
1:02.70 |
DQ |
- |
|
Arulentiran, Thanushvene (10)
|
GUGIST |
|
DQ |
- |
|
Pope, Ra'niya G (9)
|
GUGIST |
|
DQ |
- |
|
Coward, Kayin S (10)
|
GUHCAP |
|
DQ |
- |
|
Menifee, Mikinzi G (10)
|
GUHCAP |
49.97 |
DQ |
- |
|
Rodriguez, Bridget A (10)
|
GUHCAP |
55.42 |
DQ |
- |
|
Dawlett, Natalie M (9)
|
GUSSS |
|
NS |
- |