| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zhou, Katelynn K (11)
|
NTMTRO |
|
2:24.49 |
- |
| 2 |
Lonergan, Avery P (11)
|
NTUNAT |
2:32.99 |
2:30.40 |
-2.59 |
| 3 |
Nichols, Ella K (12)
|
NTESP |
2:36.57 |
2:32.04 |
-4.53 |
| 4 |
Duke, Addison E (12)
|
NTCOPS |
2:37.05 |
2:34.64 |
-2.41 |
| 5 |
Harris, Rhiannon Q (12)
|
NTCOR |
2:31.95 |
2:34.88 |
2.93 |
| 6 |
Nguyen, Anh K (12)
|
NTCOPS |
|
2:35.04 |
- |
| 7 |
Hu, Grace L (11)
|
NTMTRO |
|
2:36.28 |
- |
| 8 |
Tucker, Caroline G (11)
|
NTCOR |
|
2:39.49 |
- |
| 9 |
Crawford, Addison L (12)
|
NTMTRO |
2:38.33 |
2:40.39 |
2.06 |
| 10 |
Zhao, Cynthia M (12)
|
NTSTAR |
|
2:47.81 |
- |
| 11 |
Story, Kennedy G (11)
|
NTCOPS |
|
2:47.83 |
- |
| 12 |
De Ynchausti, Chloe D (11)
|
NTMTRO |
|
2:49.91 |
- |
| 13 |
Nishizaki, Mari (12)
|
NTCOPS |
|
2:50.12 |
- |
| 14 |
Vaughan, Libby M (11)
|
NTFNW |
3:02.16 |
2:50.54 |
-11.62 |
| 15 |
Mohsenin, Elia F (12)
|
NTMTRO |
2:47.08 |
2:52.69 |
5.61 |
| 16 |
Seaman, Sofia L (11)
|
NTCOPS |
2:59.39 |
2:53.66 |
-5.73 |
| 17 |
Evans, Samantha C (11)
|
NTMTRO |
|
2:56.87 |
- |
| 18 |
Hargraves, Alexandria R (11)
|
NTMTRO |
2:54.28 |
2:58.61 |
4.33 |
| 19 |
Ellis, Caroline G (12)
|
NTESP |
|
3:03.64 |
- |
| 20 |
Anbu, Poornima (11)
|
NTO2 |
|
3:17.24 |
- |
|
Hibbard, Maya L (11)
|
NTMTRO |
|
DQ |
- |
|
Rovik, Christina C (12)
|
NTMTRO |
3:20.82 |
NS |
- |