| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Binsfeld, Reid (10)
|
ADALBS |
36.07 |
35.99 |
-0.08 |
| 2 |
Witting, Liam T (9)
|
ADDELM |
34.72 |
36.28 |
1.56 |
| 3 |
Zeliph, Thomas J (9)
|
ADALBS |
36.77 |
37.43 |
0.66 |
| 4 |
Mistler, Bruce M (9)
|
ADSIDN |
38.96 |
38.97 |
0.01 |
| 5 |
LaFountain, Tobias P (10)
|
ADALBS |
47.05 |
39.77 |
-7.28 |
| 6 |
Nesbit, Gabriel G (10)
|
ADBENN |
43.66 |
42.59 |
-1.07 |
| 7 |
Harp, Kaden J (9)
|
ADHAWK |
43.01 |
43.24 |
0.23 |
| 8 |
Ryan, Aidan E (9)
|
ADALBS |
41.53 |
44.54 |
3.01 |
| 9 |
Murphy, James C (10)
|
ADBENN |
46.34 |
46.77 |
0.43 |
| 10 |
Vudayagiri, Sreeram (10)
|
ADBFIN |
|
50.22 |
- |
| 11 |
Heidbreder, Evan W (10)
|
ADBFIN |
|
50.66 |
- |
| 12 |
LaFountain, Jackson P (9)
|
ADALBS |
50.43 |
51.44 |
1.01 |
| 13 |
Menezes, Kyle C (9)
|
ADHAWK |
55.25 |
52.17 |
-3.08 |
| 14 |
Zeh, Ethan G (9)
|
ADBFIN |
|
52.85 |
- |
|
Waite, Liam B (10)
|
ADBFIN |
|
DQ |
- |
|
Cable, Benjamin G (9)
|
ADDELM |
41.98 |
NS |
- |