| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shao, Emma X (8)
|
ADGUY |
44.59 |
42.83 |
-1.76 |
| 2 |
Braun, Julia L (8)
|
ADNTS |
50.93 |
51.87 |
0.94 |
| 3 |
Relyea, Cadence G (8)
|
ADDELM |
1:02.40 |
52.08 |
-10.32 |
| 4 |
Cho, Anna Y (8)
|
ADALBS |
|
52.18 |
- |
| 5 |
Adams, Michelle E (7)
|
ADCPP |
59.25 |
52.24 |
-7.01 |
| 6 |
Ryan, Callie J (8)
|
ADUSAM |
54.32 |
52.34 |
-1.98 |
| 7 |
Stewart Fraser, Thornton R (8)
|
ADALBS |
1:01.14 |
52.54 |
-8.60 |
| 8 |
Sancilio, Elizabeth G (8)
|
ADDELM |
|
52.57 |
- |
| 9 |
Ryan, Chloe M (8)
|
ADUSAM |
59.68 |
54.88 |
-4.80 |
| 10 |
Colman, Maya K (8)
|
ADUN |
58.97 |
56.21 |
-2.76 |
| 11 |
Wultsch-Fuller, Sonja E (8)
|
ADNTS |
1:01.92 |
57.36 |
-4.56 |
| 12 |
Zhang, Annabelle Y (8)
|
ADCPP |
52.97 |
57.70 |
4.73 |
| 13 |
Snow, Adalynn D (7)
|
AMUN |
1:02.63 |
59.35 |
-3.28 |
| 14 |
Holden, Alexandra E (8)
|
ADCPP |
1:02.25 |
59.65 |
-2.60 |
| 15 |
Sekelj, Ella S (7)
|
ADNTS |
1:06.29 |
1:11.66 |
5.37 |
| 16 |
MacLeod, Addison M (8)
|
ADUSAM |
1:10.61 |
1:11.71 |
1.10 |
| 17 |
Carroll, Sarah J (8)
|
ADUN |
1:14.16 |
1:12.98 |
-1.18 |
|
Miller, Isabella M (8)
|
ADCPP |
|
DQ |
- |
|
Cooke, Linnea J (8)
|
ADALBS |
|
NS |
- |