| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Green, Michael R (14)
|
NTMTRO |
|
28.01 |
- |
| 2 |
Baker, Griffin B (14)
|
NTTRAC |
28.03 |
28.15 |
0.12 |
| 3 |
Smith, Jordan C (14)
|
NTTRAC |
36.99 |
28.86 |
-8.13 |
| 4 |
Ayala, Ubaldo (14)
|
UNAT |
29.25 |
28.98 |
-0.27 |
| 5 |
Szarvas, Gergely M (14)
|
LACOSS |
33.35 |
30.16 |
-3.19 |
| 5 |
Cleveland, Ryan L (14)
|
NTTRAC |
33.46 |
30.16 |
-3.30 |
| 7 |
Sullivan, Sean M (14)
|
GUAMBU |
30.87 |
30.97 |
0.10 |
| 8 |
Joseph, Caleb A (13)
|
LARRAC |
31.88 |
31.69 |
-0.19 |
| 9 |
Rhoades, Marcus A (13)
|
LARRAC |
32.09 |
32.33 |
0.24 |
| 10 |
Yarbrough, Grayson C (14)
|
NTMTRO |
|
32.45 |
- |
| 11 |
Cordell, Cooper B (13)
|
NTTRAC |
|
33.64 |
- |
| 12 |
Beggs, Christian M (13)
|
NTTRAC |
|
34.82 |
- |
| 13 |
Buenaventura, Braxton J (13)
|
NTTRAC |
37.14 |
37.84 |
0.70 |
| 14 |
Story, Christian R (13)
|
NTTRAC |
|
40.74 |
- |
| 15 |
Feiden, Cole C (13)
|
NTMTRO |
|
42.53 |
- |
| 16 |
Billington, Isaac A (13)
|
NTTRAC |
|
42.64 |
- |