| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ben-Adi, Yael (10)
|
NJTWST |
44.10 |
43.99 |
-0.11 |
| 2 |
Nugent, Aiden S (10)
|
NJSCAR |
44.72 |
45.50 |
0.78 |
| 3 |
Natsvlishvili, Matthew (10)
|
NJSCAR |
44.03 |
45.58 |
1.55 |
| 4 |
Lad, Veer A (10)
|
NJSCAR |
45.03 |
46.64 |
1.61 |
| 5 |
Gupta, Avi (9)
|
NJSCAR |
44.97 |
46.75 |
1.78 |
| 6 |
Macalintal, Zachary G (10)
|
NJSCAR |
47.24 |
46.89 |
-0.35 |
| 7 |
Kim, Benjamin S (10)
|
NJJG |
48.02 |
47.04 |
-0.98 |
| 8 |
Borgil, Bilgoon (10)
|
NJEAG |
44.86 |
47.24 |
2.38 |
| 9 |
Ludzki, Kyle (10)
|
NJSKYY |
47.45 |
47.35 |
-0.10 |
| 10 |
Lad, Jeet A (10)
|
NJSCAR |
46.45 |
48.33 |
1.88 |
| 11 |
Rhee, Jeremiah (8)
|
NJSCAR |
48.11 |
49.20 |
1.09 |
|
Zhu, James T (10)
|
NJSCAR |
47.72 |
DQ |
- |
|
Wong, Aaron H (10)
|
NJSCAR |
44.97 |
NS |
- |