| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Walborn, Mabel R (10)
|
MAUN-5 |
40.22 |
38.39 |
-1.83 |
| 1 |
Giryanskaya, Margarita (10)
|
MANLAC |
|
40.78 |
- |
| 2 |
Dixson, Caylynn E (9)
|
MAUN-3 |
45.27 |
43.30 |
-1.97 |
| 3 |
Trego, Natalie K (9)
|
MANLAC |
48.81 |
46.43 |
-2.38 |
| 4 |
Fuller, Claire M (10)
|
MANLAC |
47.71 |
47.19 |
-0.52 |
| 5 |
Prentice, Samantha K (9)
|
MAPENN |
|
54.73 |
- |
| 6 |
Mattiacci, Deanna M (10)
|
MAPENN |
|
56.51 |
- |
| 7 |
Case, Teagan L (10)
|
MANLAC |
|
57.74 |
- |
| 8 |
Cresko, Iris (10)
|
MAWASC |
|
58.82 |
- |
| 9 |
Masters, Katherine H (10)
|
MANLAC |
|
1:08.45 |
- |
|
Lukac, Cora G (10)
|
MANLAC |
55.32 |
NS |
- |
|
Howell, Samantha L (9)
|
MAWASC |
|
NS |
- |