| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wittenberg, Tori A (8)
|
MAOCCC |
47.50 |
39.09 |
-8.41 |
| 2 |
Ryan, Maxalyn E (7)
|
MADST |
42.44 |
41.76 |
-0.68 |
| 3 |
Phillips, Alexandra J (8)
|
MADST |
51.53 |
42.49 |
-9.04 |
| 4 |
Dennis, Laken B (7)
|
MAMARA |
51.97 |
44.24 |
-7.73 |
| 5 |
Radziewicz, Gabriella E (8)
|
MADST |
48.59 |
44.56 |
-4.03 |
| 6 |
Cong, Brianna A (8)
|
MADST |
|
47.85 |
- |
| 7 |
Schlosser, Ava G (7)
|
MADST |
1:05.33 |
52.84 |
-12.49 |
| 8 |
O'Donoghue, Rowan B (8)
|
MADST |
1:04.97 |
53.57 |
-11.40 |
| 9 |
Chapman, Carly J (8)
|
MADST |
1:01.67 |
54.59 |
-7.08 |
| 10 |
Long, Grace N (8)
|
MADST |
56.48 |
55.40 |
-1.08 |
|
Agrawal, Ria (8)
|
MADST |
|
DQ |
- |
|
Curtis, Catherine P (7)
|
MADST |
1:18.15 |
NS |
- |