| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
LI, Angelina (10)
|
AMANSC |
2:45.75 |
2:40.63 |
-5.12 |
| 2 |
Kunkle, Leah G (10)
|
AMFAST |
2:35.45 |
2:41.25 |
5.80 |
| 3 |
Tengowski, Julia R (10)
|
AMANSC |
3:02.17 |
2:57.27 |
-4.90 |
| 4 |
Tang, Ellie Y (10)
|
AMANSC |
3:03.73 |
3:08.69 |
4.96 |
| 5 |
Schork, Samantha N (10)
|
AMGPAC |
3:08.21 |
3:08.97 |
0.76 |
| 6 |
Zakrzewski, Brinley C (10)
|
AMWAAC |
3:18.02 |
3:12.31 |
-5.71 |
| 7 |
McCarthy, Morgan M (10)
|
AMWAAC |
3:36.91 |
3:39.09 |
2.18 |
| 8 |
Barnes, Cloey U (10)
|
AMSFAC |
3:30.21 |
3:40.52 |
10.31 |
|
Baiano, Emalina L (9)
|
AMSFAC |
4:02.19 |
NS |
- |
|
Brumbaugh, Lillian L (10)
|
AMWAAC |
4:00.00 |
NS |
- |