| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Miller, Eliza J (15)
|
AMCSC |
2:22.52 |
2:19.19 |
-3.33 |
| 2 |
Double, Natalie I (17)
|
AMSRST |
2:26.00 |
2:25.15 |
-0.85 |
| 3 |
Schomaker, Allison H (13)
|
AMPRA |
2:26.22 |
2:26.78 |
0.56 |
| 4 |
Plank, Cora J (14)
|
AMSRST |
2:27.74 |
2:27.36 |
-0.38 |
| 5 |
Morrissey, Kelly A (14)
|
AMGPAC |
2:20.11 |
2:28.32 |
8.21 |
| 6 |
Conwell, Natalie G (14)
|
AMWHAT |
2:32.90 |
2:32.49 |
-0.41 |
| 7 |
Chelli, Kaysia M (15)
|
AMSASC |
2:34.79 |
2:34.47 |
-0.32 |
| 8 |
Tursky, Addison G (14)
|
AMCSC |
2:44.56 |
2:35.46 |
-9.10 |
| 9 |
Obara, Julia R (13)
|
AMEFSC |
2:48.91 |
2:36.96 |
-11.95 |
| 10 |
Alessio, Madison A (15)
|
AMEFSC |
2:37.03 |
2:37.84 |
0.81 |
| 11 |
Plank, Anna R (16)
|
AMSRST |
2:19.48 |
2:38.22 |
18.74 |
| 12 |
Grossman, Avery R (13)
|
AMSRST |
2:46.23 |
2:39.43 |
-6.80 |
| 13 |
Burgess, Rebecca A (13)
|
AMFAST |
3:22.11 |
2:42.97 |
-39.14 |
| 14 |
DeMarco, Isabelle R (13)
|
AMSRST |
3:05.29 |
2:44.41 |
-20.88 |
| 15 |
Schwendemann, Lola P (13)
|
AMSVSC |
2:41.53 |
2:45.58 |
4.05 |
| 16 |
Josephson, Mara A (13)
|
AMSVSC |
3:05.00 |
2:46.00 |
-19.00 |
| 17 |
Verkleeren, Emma J (14)
|
AMGPAC |
2:52.85 |
2:49.13 |
-3.72 |
| 18 |
Molinero, Adalynn G (13)
|
AMSVSC |
2:46.12 |
2:50.02 |
3.90 |
| 19 |
Herron, Aubrey R (13)
|
AMSRST |
3:01.57 |
2:52.64 |
-8.93 |
| 20 |
Durec, Kendra R (14)
|
AMSASC |
3:00.23 |
2:53.25 |
-6.98 |
| 21 |
Schnur, Lily E (13)
|
AMSVSC |
3:04.91 |
3:05.91 |
1.00 |
| 22 |
Kane, Taylor L (14)
|
AMPRA |
3:35.56 |
3:12.48 |
-23.08 |
|
Giazzoni, Mary E (13)
|
AMSASC |
3:15.87 |
DQ |
- |