| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kunkle, Leah G (12)
|
AMFAST |
2:25.57 |
2:25.36 |
-0.21 |
| 2 |
Bookwalter, Katelyn E (12)
|
AMSVSC |
2:28.96 |
2:30.74 |
1.78 |
| 3 |
Freshcorn, Rylee J (11)
|
AM3RVS |
2:58.33 |
2:41.66 |
-16.67 |
| 4 |
Sasso, Aviana G (12)
|
AMRXA |
2:50.43 |
2:42.79 |
-7.64 |
| 5 |
Daniele, Anabella N (12)
|
AMSVSC |
2:52.34 |
2:44.97 |
-7.37 |
| 6 |
Keeley, Zoey A (12)
|
AMWAAC |
3:10.34 |
2:49.01 |
-21.33 |
| 7 |
Biltz, Avery M (12)
|
AMCSC |
2:54.56 |
2:50.97 |
-3.59 |
| 8 |
Gregorowicz, Caroline O (12)
|
AMPRA |
3:11.47 |
2:58.44 |
-13.03 |
| 9 |
Iddings, Audra L (11)
|
AMWHAT |
2:55.69 |
3:02.37 |
6.68 |
| 10 |
Mansfield, Kendall L (12)
|
AMSVSC |
3:40.57 |
3:38.90 |
-1.67 |
| 11 |
Kratz, Abigail M (11)
|
AM3RVS |
3:50.00 |
3:50.51 |
0.51 |
|
Goughnour, Laine M (11)
|
AMWHAT |
3:10.69 |
DQ |
- |