| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lewis, Christopher J (10)
|
NIQUIK |
48.20 |
50.10 |
1.90 |
| 2 |
Parsnick, William Z (8)
|
NICLSC |
54.45 |
50.60 |
-3.85 |
| 3 |
Stahl, Reed P (10)
|
NIVICT |
49.55 |
50.72 |
1.17 |
| 4 |
Shields, Zachary W (10)
|
NIVICT |
54.73 |
52.42 |
-2.31 |
| 5 |
Battaglia, Benjamin P (8)
|
NIVICT |
56.22 |
55.61 |
-0.61 |
| 6 |
Bajus, Nathan J (10)
|
NIVICT |
55.68 |
56.74 |
1.06 |
| 7 |
Ashby, Jacob T (9)
|
NISDSC |
1:01.17 |
57.53 |
-3.64 |
| 8 |
Gucker, John W (10)
|
NIRRST |
|
58.81 |
- |
|
He, Andrew (10)
|
NIVICT |
57.83 |
57.14 |
-0.69 |
|
Pogroszewski, Noah Z (10)
|
NIGCOM |
|
DQ |
- |
|
Kendall, Logan F (10)
|
NIMERC |
47.80 |
DQ |
- |
|
Zeller, Matthew R (9)
|
NISDSC |
|
DQ |
- |
|
Harris, Wesley S (8)
|
NIVICT |
|
DQ |
- |