| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Henshaw, Emerson F (12)
|
VA757 |
3:19.68 |
3:02.46 |
-17.22 |
| 2 |
Landon, Sarah K (11)
|
VAECAT |
3:16.67 |
3:12.07 |
-4.60 |
| 3 |
Heustis, Brooke A (12)
|
VAODAC |
3:20.27 |
3:16.13 |
-4.14 |
| 4 |
Mueller, Taylor N (11)
|
VACGBD |
3:25.39 |
3:19.69 |
-5.70 |
| 5 |
Swartzbaugh, Anna M (11)
|
VAODAC |
3:28.21 |
3:24.52 |
-3.69 |
| 6 |
Peterson, Campbell M (12)
|
VATIDE |
3:17.12 |
3:25.41 |
8.29 |
| 7 |
Kirkpatrick, Caeleigh A (12)
|
VATIDE |
3:52.77 |
3:51.54 |
-1.23 |
| 8 |
Cunningham, Claire M (12)
|
VATIDE |
3:48.77 |
3:54.95 |
6.18 |
| 9 |
Deputy, Ashlynn G (12)
|
VATIDE |
|
4:02.75 |
- |
| 10 |
Mireles, Madeline R (12)
|
VAODAC |
4:08.39 |
4:12.78 |
4.39 |
|
Reitzel, Gianna P (12)
|
VATIDE |
3:38.34 |
DQ |
- |
|
Hamilton, Tessa E (12)
|
VATIDE |
3:41.38 |
NS |
- |