| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Simmons, Carly (6)
|
MAT85 |
26.33 |
24.48 |
-1.85 |
| 2 |
Ward, Maya L (6)
|
MAWAC |
|
25.34 |
- |
| 3 |
Hauske, Megan L (6)
|
MAWAC |
30.99 |
25.91 |
-5.08 |
| 4 |
Holcroft, Lucy L (6)
|
MASJAC |
|
26.27 |
- |
| 5 |
Pasquini, Aubrey E (6)
|
MASJAC |
|
26.71 |
- |
| 6 |
Grant, Jayden C (6)
|
MAWAC |
29.65 |
27.35 |
-2.30 |
| 7 |
Gurcik, Alyssa F (6)
|
MASJAC |
|
27.41 |
- |
| 8 |
Martino, Alyssa (6)
|
MAT85 |
|
27.77 |
- |
| 9 |
Krutoff, Stella J (6)
|
MASJAC |
|
28.19 |
- |
| 10 |
Jauffret, Clementine F (6)
|
MAWAC |
|
28.25 |
- |
| 11 |
Varani, Tessa P (6)
|
MATHY |
|
29.12 |
- |
| 12 |
Gaynor, Juliet E (6)
|
MASJAC |
|
29.46 |
- |
| 13 |
O'Brien, Emma R (6)
|
MAWAC |
|
29.93 |
- |
| 14 |
Weag, Emilia C (6)
|
MASJAC |
|
32.29 |
- |
| 15 |
Gratten, Sophia J (5)
|
MAPENN |
|
36.37 |
- |
| 16 |
Gateretse, Isabelle L (4)
|
MAWAC |
39.35 |
39.04 |
-0.31 |
| 17 |
Giordano, Charlotte M (5)
|
MAWAC |
|
47.92 |
- |
|
Alberto, Emerson J (5)
|
MAWAC |
1:08.82 |
DQ |
- |
|
Kantner, Madelyn O (5)
|
MAWAC |
|
DQ |
- |