| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rodriguez, Neomi (8)
|
MANSC |
|
41.43 |
- |
| 2 |
Kiesel, Addison E (8)
|
MASJAC |
48.54 |
42.10 |
-6.44 |
| 3 |
McKendrick, Ainsley K (8)
|
MATWAC |
43.33 |
42.51 |
-0.82 |
| 4 |
Bontrager, Selah G (8)
|
MASJAC |
|
45.87 |
- |
| 5 |
Crouthamel, Lauren C (8)
|
MAWWC |
53.17 |
46.31 |
-6.86 |
| 6 |
Petrillo, Jenna A (8)
|
MATWAC |
|
48.05 |
- |
| 7 |
Hauske, Sara A (8)
|
MAWAC |
1:05.30 |
55.74 |
-9.56 |
| 8 |
Rice, Addison (8)
|
MAWAC |
|
55.77 |
- |
| 9 |
Poole, Kylie M (7)
|
MAWAC |
|
58.11 |
- |
| 10 |
Curtis, Mia S (8)
|
MAWAC |
|
1:05.28 |
- |
| 11 |
Hauske, Katelyn M (8)
|
MAWAC |
1:09.29 |
1:08.22 |
-1.07 |
| 12 |
Carroll, Teagan R (8)
|
MASTRM |
1:12.98 |
1:13.95 |
0.97 |
| 13 |
Morrow, Kylie E (7)
|
MAWAC |
|
1:19.85 |
- |
| 14 |
Cruse, Vivian L (8)
|
MASJAC |
|
1:20.02 |
- |
|
Shadman, Sydney E (8)
|
MASTRM |
1:21.33 |
DQ |
- |
|
Nunez-Ledesma, Amelie (8)
|
MANSC |
1:08.42 |
DQ |
- |