| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kelsey, Peyton M (6)
|
UNAT |
25.74 |
23.29 |
-2.45 |
| 2 |
Devlin, Keira E (6)
|
MAWAC |
26.44 |
24.08 |
-2.36 |
| 3 |
Hutton, Ellie R (6)
|
MAWAC |
26.81 |
24.83 |
-1.98 |
| 4 |
Resnick, Reese B (6)
|
MAECCC |
27.74 |
27.11 |
-0.63 |
| 5 |
Wainwright, Ella G (6)
|
MAECCC |
31.51 |
27.17 |
-4.34 |
| 6 |
Scott, Julia T (5)
|
MASJAC |
28.57 |
27.51 |
-1.06 |
| 7 |
Smith, Aurora G (6)
|
MAECCC |
36.23 |
29.19 |
-7.04 |
| 8 |
Grant, Jayden C (6)
|
MAWAC |
34.57 |
29.65 |
-4.92 |
| 9 |
Shur, Brenna R (6)
|
MASEA |
28.68 |
29.84 |
1.16 |
| 10 |
Hauske, Megan L (5)
|
MAWAC |
35.36 |
30.99 |
-4.37 |
| 11 |
Smith, Tristyn S (5)
|
MAGPAC |
33.54 |
32.47 |
-1.07 |
| 12 |
Boyer, Emma L (6)
|
MAWAC |
33.32 |
34.93 |
1.61 |
| 13 |
Hellmann, Anneliese M (6)
|
MAPRSC |
34.69 |
35.55 |
0.86 |
| 14 |
Emmett, Reese A (6)
|
MAGPAC |
34.47 |
35.57 |
1.10 |
| 15 |
Bluemle, Greta J (6)
|
MAWAC |
37.81 |
35.84 |
-1.97 |
| 16 |
DeRogatis, Leah E (6)
|
MAPRSC |
55.00 |
41.03 |
-13.97 |
| 17 |
Gateretse, Isabelle L (4)
|
MAWAC |
39.35 |
41.96 |
2.61 |