| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Norton, Jasmine E (12)
|
MANPAC |
36.53 |
35.56 |
-0.97 |
| 2 |
Manning, Jazziah A (12)
|
MADCAA |
|
38.55 |
- |
| 3 |
Stevenson, Abigail (11)
|
MAGSA |
39.64 |
38.84 |
-0.80 |
| 4 |
Cicco, Emily E (11)
|
MAGSA |
39.50 |
39.06 |
-0.44 |
| 5 |
Pennekamp, Eleanora L (12)
|
MADCAA |
40.13 |
40.37 |
0.24 |
| 6 |
Nash, Hannah E (12)
|
MADCAA |
|
40.62 |
- |
| 7 |
Truitt, Zoe (11)
|
MANSC |
41.16 |
41.66 |
0.50 |
| 8 |
Welsh, Zoe C (11)
|
MANPAC |
41.96 |
41.84 |
-0.12 |
| 9 |
Wittmer, Sarah A (12)
|
MAGSA |
41.60 |
42.77 |
1.17 |
| 10 |
Bueno, Nathalia E (11)
|
MANSC |
|
43.53 |
- |
| 11 |
Descher, Carol Ann (11)
|
MANSC |
43.02 |
43.58 |
0.56 |
| 12 |
Garrabrant, Megan S (12)
|
MAGSA |
43.23 |
44.94 |
1.71 |
| 13 |
McNeely, Michelle (12)
|
MANSC |
49.88 |
48.90 |
-0.98 |
| 14 |
Cohen, Rachel E (11)
|
MAGSA |
50.01 |
49.26 |
-0.75 |
| 15 |
Bates, Ciara J (12)
|
MAGSA |
49.20 |
49.91 |
0.71 |
| 16 |
Rodriguez-Alevedo, Ayaira N (11)
|
MANSC |
1:00.41 |
58.20 |
-2.21 |
| 17 |
Eibel, Brigid M (11)
|
MANSC |
|
1:00.02 |
- |
|
Ruiz, Paullina (12)
|
MANSC |
|
NS |
- |