| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Holzer, Avery G (7)
|
MAECCC |
48.93 |
48.82 |
-0.11 |
| 2 |
Gulli, Kendall J (7)
|
MAWAC |
51.85 |
49.81 |
-2.04 |
| 3 |
Schultz, Gretchen E (7)
|
MAECCC |
54.28 |
52.76 |
-1.52 |
| 4 |
Stanker, Rhiannon G (7)
|
MAGPAC |
51.84 |
53.31 |
1.47 |
| 5 |
Horsey, Riley E (7)
|
MAWAC |
59.08 |
53.60 |
-5.48 |
| 6 |
Sheehan, Shaelin J (7)
|
MAJW |
1:15.24 |
54.72 |
-20.52 |
| 7 |
Procida, Avery M (7)
|
MAGPAC |
1:00.42 |
55.20 |
-5.22 |
| 8 |
Kalinowski, Lillian R (7)
|
MAWAC |
1:01.31 |
58.64 |
-2.67 |
| 9 |
Brost, Ruby A (7)
|
MAWWC |
1:06.43 |
1:00.71 |
-5.72 |
| 10 |
Briscoe, Chloe R (7)
|
MAGPAC |
1:02.10 |
1:01.66 |
-0.44 |
| 11 |
Kowaleski, Calliope D (7)
|
MASEA |
1:11.70 |
1:04.72 |
-6.98 |
| 12 |
Thoensen, Avari (7)
|
MASEA |
1:20.55 |
1:05.47 |
-15.08 |
| 13 |
Davidson, Taylor A (7)
|
MAECCC |
1:13.55 |
1:06.32 |
-7.23 |
| 14 |
Ruslim, Eva (7)
|
MAWAC |
1:11.94 |
1:06.72 |
-5.22 |
| 15 |
Mitchell, Maura (7)
|
MASJAC |
1:17.71 |
1:07.64 |
-10.07 |
| 16 |
Jones, Madeleine H (7)
|
MAWAC |
1:06.89 |
1:08.83 |
1.94 |
| 17 |
Morrow, Kylie E (7)
|
MAWAC |
1:13.80 |
1:11.14 |
-2.66 |
| 18 |
Headrick, Lucy F (7)
|
MASJAC |
1:10.14 |
1:12.49 |
2.35 |
| 19 |
Bieg, Theresa B (7)
|
MASJAC |
1:19.65 |
1:13.92 |
-5.73 |
| 20 |
Jubb, Penelope E (7)
|
MAWAC |
1:30.12 |
1:19.10 |
-11.02 |