| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ying, Allan Z (10)
|
MDCAA |
49.76 |
45.92 |
-3.84 |
| 2 |
Duncan, Langston (10)
|
MDCAA |
54.75 |
46.33 |
-8.42 |
| 3 |
Spencer, Sebastien G (10)
|
MDCAA |
|
46.49 |
- |
| 4 |
Di Gregorio, Caleb E (10)
|
MDMAS |
53.92 |
50.65 |
-3.27 |
| 5 |
Frank, Jonathan J (10)
|
MDCAA |
57.17 |
50.82 |
-6.35 |
| 6 |
Johnson, Brayden C (10)
|
MDCAA |
59.06 |
51.28 |
-7.78 |
| 7 |
Cui, Nicholas (10)
|
MDEST |
56.38 |
52.10 |
-4.28 |
| 8 |
Vandiver, Jack M (10)
|
MDMAS |
|
53.47 |
- |
| 9 |
Kotlyarskiy, Phillip A (10)
|
MDJCC |
57.15 |
54.86 |
-2.29 |
| 10 |
Wu, Matthew Y (10)
|
MDCAA |
|
54.99 |
- |
| 11 |
Megdiche, Ryan (10)
|
MDCAA |
|
55.45 |
- |
| 12 |
LeGuellec, Mewenn (10)
|
MDMAC |
59.45 |
56.26 |
-3.19 |
| 13 |
Eisenhardt, Leif J (10)
|
MDMAS |
|
56.72 |
- |
| 14 |
Williams, Landon D (10)
|
MDFAST |
|
56.85 |
- |
| 15 |
Bala, Charan S (10)
|
MDEST |
1:06.81 |
1:06.71 |
-0.10 |
| 16 |
Longo, James P (10)
|
MDHSST |
|
1:07.95 |
- |
|
Siri, Braden W (10)
|
MDMAS |
48.49 |
NS |
- |