| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Good, Matthew M (9)
|
MDFAST |
45.12 |
43.40 |
-1.72 |
| 2 |
Pustavoitau, Zachary A (9)
|
MDEST |
50.37 |
45.15 |
-5.22 |
| 3 |
Obasohan, Nicholas E (9)
|
MDFAST |
47.30 |
45.81 |
-1.49 |
| 4 |
Berrios, Kaito V (9)
|
MDEST |
1:01.76 |
46.15 |
-15.61 |
| 5 |
Dragic, Nikola A (9)
|
MDFAST |
1:12.08 |
48.37 |
-23.71 |
| 6 |
Linstrom, Brian J (9)
|
MDCAA |
59.69 |
49.23 |
-10.46 |
| 7 |
Panfiorau, Anthony V (9)
|
MDEST |
50.95 |
49.34 |
-1.61 |
| 8 |
Chiang, Lucas H (9)
|
MDCAA |
1:06.73 |
49.56 |
-17.17 |
| 9 |
Cosgrove, Ryan J (9)
|
MDMAC |
51.91 |
49.59 |
-2.32 |
| 10 |
Zhong, Aaron T (9)
|
MDCAA |
51.07 |
49.68 |
-1.39 |
| 11 |
Nettles, Jai E (9)
|
MDEST |
47.78 |
50.80 |
3.02 |
| 12 |
Agboli, Ezra I (9)
|
MDCAA |
|
51.46 |
- |
| 13 |
Matsumoto, Keita (9)
|
MDJCC |
49.71 |
51.64 |
1.93 |
| 14 |
Li, Jerry E (9)
|
MDCAA |
51.96 |
52.47 |
0.51 |
| 15 |
Dolezel, Dominik M (9)
|
MDCAA |
|
52.76 |
- |
| 16 |
Reinhard, Cameron (9)
|
MDRAC |
|
53.02 |
- |
| 17 |
Varacalle-Goetz, Eyan C (9)
|
MDHSST |
54.59 |
53.66 |
-0.93 |
| 18 |
Byrne, Wyatt M (9)
|
MDFSC |
1:02.90 |
53.68 |
-9.22 |
| 19 |
Liu, William T (9)
|
MDMAC |
49.82 |
54.26 |
4.44 |
| 20 |
Dukes-Robinson, Christopher (9)
|
MDBCSC |
1:07.15 |
56.18 |
-10.97 |
| 21 |
Linstrom, Kevin J (9)
|
MDCAA |
|
57.42 |
- |
| 22 |
Nash, Brody A (9)
|
MDHSST |
|
1:00.06 |
- |
| 23 |
Jackson, Chase A (9)
|
MDFSC |
|
1:06.11 |
- |
|
Woldeselassie, David T (9)
|
MDEST |
51.44 |
NS |
- |
|
Gheiler, Ilan (9)
|
MDJCC |
1:04.44 |
NS |
- |
|
Young, Evan J (9)
|
MDHSST |
1:06.87 |
NS |
- |