| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Winters, Taylor L (9)
|
MDFSC |
49.49 |
46.86 |
-2.63 |
| 2 |
Cowley, Peyton E (9)
|
MDEST |
48.25 |
47.16 |
-1.09 |
| 3 |
DeGross, Carrington M (9)
|
MDBCSC |
48.60 |
47.52 |
-1.08 |
| 4 |
Ekanayake, Nihara T (9)
|
MDEST |
51.60 |
48.67 |
-2.93 |
| 5 |
Wang, Eliana Y (9)
|
MDCAA |
52.25 |
50.29 |
-1.96 |
| 6 |
Brissett, Gabrielle S (9)
|
MDEST |
54.38 |
50.95 |
-3.43 |
| 7 |
Santos, Olivia H (9)
|
MDCAA |
50.02 |
51.57 |
1.55 |
| 8 |
Babik, Finley E (9)
|
MDCAA |
53.15 |
51.97 |
-1.18 |
| 10 |
Harrington, Jordan H (9)
|
MDCAA |
55.91 |
55.63 |
-0.28 |
| 12 |
Wollenweber, Elliana S (9)
|
MDEST |
1:02.65 |
59.17 |
-3.48 |
| 13 |
Vicinelly, Alina M (9)
|
MDFSC |
57.86 |
1:00.60 |
2.74 |
| 14 |
Inskeep, Iris N (9)
|
MDFAST |
|
1:01.59 |
- |
| 16 |
McGee, Kyleigh J (9)
|
MDFSC |
|
1:04.83 |
- |
| 17 |
Moore, Harper C (9)
|
MDFSC |
1:11.10 |
1:11.31 |
0.21 |
| 18 |
Stitt, Olivia L (9)
|
MDCAA |
|
1:20.45 |
- |
|
Chen, Grace Z (9)
|
MDCAA |
|
DQ |
- |
|
Edwards, Keola M (9)
|
MDBCSC |
|
DQ |
- |