| # | Swimmer | Team | Seed | Time | Diff |
| 2 |
Niles, Riley L (8)
|
MDEST |
17.46 |
17.08 |
-0.38 |
| 3 |
Chesnokov, Alexandra (8)
|
MDEST |
17.88 |
17.15 |
-0.73 |
| 4 |
Harshman, Leah N (8)
|
MDFAST |
19.04 |
17.19 |
-1.85 |
| 5 |
Duren, Jayla T (8)
|
MDBCSC |
20.37 |
17.58 |
-2.79 |
| 6 |
Faul, Evelyn J (8)
|
MDCAA |
|
19.08 |
- |
| 7 |
Fortune, Elyana R (8)
|
MDFSC |
|
19.64 |
- |
| 9 |
Kacher, Paige H (8)
|
MDHSST |
26.90 |
20.51 |
-6.39 |
| 11 |
Almagro, Amani K (8)
|
MDCAA |
|
21.31 |
- |
| 13 |
Bugin, Alexandra S (8)
|
MDFAST |
25.28 |
21.35 |
-3.93 |
| 18 |
Chalasani, Priyanka (8)
|
MDEST |
|
25.70 |
- |
|
Morton, Za'kayah L (8)
|
MDBCSC |
21.50 |
NS |
- |
|
McDonald, Mackenzie L (8)
|
MDEST |
|
NS |
- |