| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Niles, Riley L (8)
|
MDEST |
19.20 |
19.44 |
0.24 |
| 3 |
Duren, Jayla T (8)
|
MDBCSC |
28.45 |
21.01 |
-7.44 |
| 4 |
Chesnokov, Alexandra (8)
|
MDEST |
25.45 |
21.65 |
-3.80 |
| 5 |
Harshman, Leah N (8)
|
MDFAST |
25.95 |
21.67 |
-4.28 |
| 7 |
Blaha, Sienna K (8)
|
MDEST |
22.72 |
23.05 |
0.33 |
| 8 |
Hanlon, Kelsey R (8)
|
MDFSC |
27.25 |
23.54 |
-3.71 |
| 10 |
Kacher, Paige H (8)
|
MDHSST |
25.29 |
24.15 |
-1.14 |
| 13 |
Tomlinson, Sadie R (8)
|
MDFSC |
|
27.10 |
- |
| 16 |
Almagro, Amani K (8)
|
MDCAA |
|
30.12 |
- |
|
Piche, Paige E (8)
|
MDCAA |
34.81 |
DQ |
- |
|
Chalasani, Priyanka (8)
|
MDEST |
|
DQ |
- |
|
Morton, Za'kayah L (8)
|
MDBCSC |
28.37 |
NS |
- |
|
McDonald, Mackenzie L (8)
|
MDEST |
|
NS |
- |