| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mansaray, Mia-Light (7)
|
MDCAA |
|
48.03 |
- |
| 2 |
Kundey-Reiten, Sage L (7)
|
MDMAC |
47.77 |
48.34 |
0.57 |
| 3 |
Stern, Stella E (7)
|
MDASC |
52.92 |
48.35 |
-4.57 |
| 4 |
Franciscovich, Harper J (7)
|
MDFAST |
55.41 |
49.38 |
-6.03 |
| 5 |
Knight, Lucy A (7)
|
MDEST |
50.91 |
49.46 |
-1.45 |
| 6 |
Leonard, Brielle J (7)
|
MDACA |
58.36 |
49.99 |
-8.37 |
| 7 |
Best, Remi (7)
|
MDCAA |
52.81 |
50.14 |
-2.67 |
| 8 |
Ejanda, Caitlin T (7)
|
MDRAC |
50.55 |
51.38 |
0.83 |
| 9 |
Verhoosel, Everly R (7)
|
MDASC |
|
51.63 |
- |
| 10 |
Decktor, Aurora Q (7)
|
MDEST |
|
52.46 |
- |
| 11 |
Bach, Zoya E (7)
|
MDEST |
57.27 |
52.65 |
-4.62 |
| 12 |
DeMay, Bela M (7)
|
MDASC |
|
53.02 |
- |
| 13 |
Sheehan, Hinckley M (7)
|
MDASC |
1:07.77 |
53.21 |
-14.56 |
| 14 |
Schindler, Julianna J (7)
|
MDGTAC |
54.69 |
53.61 |
-1.08 |
| 15 |
Liu, Chelsea (7)
|
MDMAC |
58.83 |
54.16 |
-4.67 |
| 16 |
Leeds, Claire E (7)
|
MDMAC |
48.95 |
54.81 |
5.86 |
| 17 |
Foster, Ayla B (7)
|
MDMAC |
57.91 |
55.68 |
-2.23 |
| 18 |
Beaver, Maeve F (7)
|
MDMAC |
1:03.05 |
55.78 |
-7.27 |
| 19 |
Cannone, Cora R (7)
|
MDASC |
58.78 |
56.45 |
-2.33 |
| 20 |
Pyzik, Grace A (7)
|
MDNBAC |
58.49 |
56.55 |
-1.94 |
| 21 |
Tigani, Leona B (7)
|
MDCAA |
|
56.63 |
- |
| 22 |
Brinson, Parker H (7)
|
MDASC |
59.88 |
58.08 |
-1.80 |
| 23 |
Ortiz, Gianna R (7)
|
MDMAC |
1:00.55 |
59.17 |
-1.38 |
| 24 |
Flores, Ariel N (7)
|
MDMAC |
|
59.75 |
- |
| 25 |
Boehmer, Madeline M (7)
|
MDEST |
1:03.51 |
1:01.06 |
-2.45 |
| 26 |
Marroquin, Penelope M (7)
|
MDMAC |
57.98 |
1:01.22 |
3.24 |
| 27 |
Espinal, Carolina D (7)
|
MDMAC |
1:01.35 |
1:03.21 |
1.86 |
| 28 |
Tarlan, Hannah (7)
|
MDFAST |
1:02.11 |
1:04.62 |
2.51 |
| 29 |
Pieninck, Ava H (7)
|
MDEST |
1:01.78 |
1:05.72 |
3.94 |
|
Wingate, Ella R (7)
|
MDEST |
53.98 |
DQ |
- |
|
Harvill, Eleanor J (7)
|
MDEST |
1:01.02 |
DQ |
- |
|
Pang, Jolina Y (7)
|
MDCAA |
1:00.16 |
DQ |
- |
|
Ward, Quinn A (7)
|
MDASC |
45.72 |
DQ |
- |