| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Alex, Kenzie M (9)
|
PVNCAP |
46.10 |
44.20 |
-1.90 |
| 2 |
Peterson, Analiese M (9)
|
VAVSTP |
46.06 |
45.08 |
-0.98 |
| 3 |
Yelverton, Samantha A (10)
|
PVNCAP |
52.92 |
48.83 |
-4.09 |
| 4 |
Fuller, Emersyn P (10)
|
PVNCAP |
49.34 |
49.78 |
0.44 |
| 5 |
Plutz, Delaney L (10)
|
PVNCAP |
53.61 |
50.16 |
-3.45 |
| 6 |
McGinley, Brooklyn L (9)
|
PVNCAP |
56.16 |
51.74 |
-4.42 |
| 7 |
Brand, Isabella H (10)
|
PVNCAP |
56.52 |
53.88 |
-2.64 |
| 8 |
Rannells, Kimberly G (10)
|
PVNCAP |
59.15 |
57.18 |
-1.97 |
| 9 |
Waffo-Gassion, Addison E (9)
|
VAPWSC |
1:12.07 |
1:04.08 |
-7.99 |
|
Roberts, Emerson K (9)
|
VATORP |
1:04.70 |
NS |
- |