| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Busato, Mya J (10)
|
PVNCAP |
40.20 |
38.16 |
-2.04 |
| 2 |
Shin, Diane J (9)
|
PVNCAP |
41.35 |
39.58 |
-1.77 |
| 3 |
Struder, Elizabeth A (10)
|
VARAYS |
43.24 |
41.79 |
-1.45 |
| 4 |
Artz, Lydia R (10)
|
VAVSTP |
52.00 |
43.67 |
-8.33 |
| 5 |
Richards, Ella M (10)
|
PVNCAP |
47.06 |
45.77 |
-1.29 |
| 6 |
Demissie, Hannah B (9)
|
PVNCAP |
46.62 |
47.67 |
1.05 |
| 7 |
Edenberg, Abigail L (10)
|
PVNCAP |
48.40 |
48.32 |
-0.08 |
| 8 |
Wood, Tenley C (10)
|
VARAYS |
54.49 |
48.60 |
-5.89 |
| 9 |
Tutkus, Austeja (10)
|
PVNCAP |
50.18 |
49.83 |
-0.35 |
| 10 |
Maphis, Olivia T (10)
|
VAVSTP |
|
50.66 |
- |
| 11 |
Shade, Brynlea E (10)
|
VAVSTP |
1:00.69 |
56.26 |
-4.43 |
| 12 |
Garcia, Charlotte R (10)
|
VARAYS |
58.95 |
1:00.94 |
1.99 |
| 13 |
Chinn, Brooklyn K (9)
|
VAVSTP |
1:05.81 |
1:01.36 |
-4.45 |
| 14 |
Combs, Kennedy M (9)
|
VAVSTP |
1:19.00 |
1:15.36 |
-3.64 |
|
Torres, Iris S (9)
|
VARAYS |
|
DQ |
- |
|
Orth, Kathryn M (10)
|
VAVSTP |
|
NS |
- |
|
Diaz Tellez, Camila A (10)
|
VAPWSC |
58.38 |
NS |
- |
|
Stanford, Soleil M (10)
|
VAVSTP |
|
NS |
- |