| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Barbour, Alexander F (9)
|
PVNCAP |
47.50 |
36.13 |
-11.37 |
| 2 |
Mara, Josua D (10)
|
PVNCAP |
37.38 |
37.70 |
0.32 |
| 3 |
Sehgal, Xander (10)
|
PVDCPR |
41.65 |
39.59 |
-2.06 |
| 4 |
Henrikson-Brandt, Kai (9)
|
PVDCPR |
42.05 |
40.09 |
-1.96 |
| 5 |
Kweon, Matthew M (10)
|
PVMAC |
44.18 |
41.16 |
-3.02 |
| 6 |
Catanzano, Max A (10)
|
PVDCPR |
42.21 |
41.51 |
-0.70 |
| 7 |
Davis Jr., Michael D (10)
|
PVDCPR |
42.95 |
41.80 |
-1.15 |
| 8 |
Lam, Tristan C (9)
|
PVDCPR |
1:05.07 |
44.96 |
-20.11 |
| 9 |
Om, Ethan (9)
|
PVMAC |
50.26 |
46.37 |
-3.89 |
| 10 |
Kim, Phillip H (9)
|
PVMAC |
46.55 |
46.57 |
0.02 |
| 11 |
Kaleem, Jacob D (9)
|
PVNCAP |
52.31 |
47.34 |
-4.97 |
| 12 |
Crawford, Lucious F (10)
|
PVDCPR |
46.66 |
47.71 |
1.05 |
| 13 |
Wilkins, Zachary T (10)
|
PVNCAP |
51.11 |
48.10 |
-3.01 |
| 14 |
Schon, Dylan (10)
|
PVDCPR |
50.17 |
50.60 |
0.43 |
| 15 |
Oakes, Finnegan K (10)
|
PVDCPR |
|
54.65 |
- |
|
Joseph, Sulaiman B (9)
|
PVDCPR |
47.30 |
NS |
- |